Extraction of Bronchodilators on Strata™ X
LC Conditions
Column
Luna 5 µm C8(2) 100 Å, LC Column 150 x 4.6 mm, Ea
Brand
Luna
Part No
00F-4249-E0
Phase Name
C8(2)
Elution Type
Isocratic
Mobile Phase
A: 20 mM KH2PO4, pH 2.5
B: ACN
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
95
5
Flow Rate
1.50 mL/min
Temperature
25°C
Detection
UV-Vis Abs.-Fixed Wave. (UV) @ (22°C)
Detection Info
Sample Notes
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C8 4 x 3.0mm, 10/Pk Part No.: AJ0-4290 - Holder Part No.: KJ0-4282
Sample Preparation Details
Description
Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk
Part No
8B-S100-TAK
Pretreatment Note
Blow down to almost dryness under slow stream of N2. Reconstitute with mobile phase to 200 uL
Condition 1
1.0 mL Methanol
Condition 2
1.0 mL Water
Load
Load 1.00 mL Sample diluted in Buffer
Wash 1
1.0 mL 5% methanol/D. I. water
Wash 2
1 at 2
Dry
for 1 min at 10'' of Hg
Elution 1
1.0 mL Methanol
Evaporation
Evaporate to dryness
Reconstitute
2 mL Mobile Phase
Order Items Used in This Application
- 1. Theobromine
- 2. Theophylline
- 3. Beta hydroxy theophylline
- 4. Caffeine
-OEChem-06211003152D<br><br> 21 22 0 0 0 0 0 0 0999 V2000<br> 3.7320 2.0000 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -1.0000 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.5443 0.8047 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -1.0000 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 0.5000 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.5443 -0.8047 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 0.5000 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 -0.5000 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 1.0000 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -0.5000 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.1279 0.0000 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -2.0000 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.8550 1.7552 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.7479 0.0000 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3291 0.8100 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.1120 -2.0000 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -2.6200 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3520 -2.0000 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.4443 1.5626 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0476 2.3446 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2656 1.9479 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 9 2 0 0 0 0<br> 2 10 2 0 0 0 0<br> 3 7 1 0 0 0 0<br> 3 11 1 0 0 0 0<br> 3 13 1 0 0 0 0<br> 4 8 1 0 0 0 0<br> 4 10 1 0 0 0 0<br> 4 12 1 0 0 0 0<br> 5 9 1 0 0 0 0<br> 5 10 1 0 0 0 0<br> 5 15 1 0 0 0 0<br> 6 8 1 0 0 0 0<br> 6 11 2 0 0 0 0<br> 7 8 2 0 0 0 0<br> 7 9 1 0 0 0 0<br> 11 14 1 0 0 0 0<br> 12 16 1 0 0 0 0<br> 12 17 1 0 0 0 0<br> 12 18 1 0 0 0 0<br> 13 19 1 0 0 0 0<br> 13 20 1 0 0 0 0<br> 13 21 1 0 0 0 0<br>M END
Compound Name
Theobromine
CID
5429
Molecular Formula
C7H8N4O2
Molecular Weight
180
No. Hydrogen Bond Acceptors
3
No. Hydrogen Bond Donors
1
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