EPA Method 553, Benzidines and Nitrogen-Containing Pesticides

LC Conditions

Official Method

EPA 553

Column

Luna 5 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4252-E0

Phase Name

C18(2)

Elution Type

Gradient

Mobile Phase

A: 10mM Ammonium Acetate:Acetonitrile (75:25)

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

75

25

2

1

75

25

3

29

40

60

4

49

40

60

Flow Rate

0.30 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282