Peptides from Serum on Strata™ X

LC Conditions

Column

Jupiter 4 µm Proteo 90 Å, LC Column 150 x 4.6 mm, Ea

Brand

Jupiter

Part No

00F-4396-E0

Phase Name

Proteo

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA

B: ACN:0.1% TFA in water (90:10)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

22

40

60

Flow Rate

1.00 mL/min

Temperature

30°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074 - Holder Part No.: KJ0-4282

Sample Preparation Details

Description

Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk

Part No

8B-S100-TAK

Pretreatment Note

EVAPORATE AND RECONSTITUTE .40 °C under nitrogen stream 300 mL 0.1% aqueous TFA

Condition 1

1.0 mL Methanol

Condition 2

1.0 mL Water

Sample Pretreatment

Strata X 33u 30 mg / 1 ml Tube Condition: 1ml Methanol Load: 1 ml Spiked porcine serum extract

Load

Load 1.00 mL Sample

Wash 1

1.0 mL 10% Acetonitrile in 0.1% TFA

Dry

1 min @ 10 in. Hg

Elution 1

1.0 mL Acetonitrile:Water

Evaporation

Evaporate to dryness

Reconstitute

300 µL TFA (0.1%) in Water