Extraction of Diazepam on Strata™ X

LC Conditions

Column

Synergi 4 µm Polar-RP 80 Å, LC Column 150 x 4.6 mm, Ea

Brand

Synergi

Part No

00F-4336-E0

Phase Name

Polar-RP

Elution Type

Gradient

Mobile Phase

A: 20 mM KH2PO4 (pH=2.5)

B: MeOH

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

75

25

2

3

75

25

3

8

30

70

4

11

30

70

5

14

75

25

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, POLAR-RP 4 x 3.0mm, 10/Pk Part No.: AJ0-6076 - Holder Part No.: KJ0-4282

Sample Preparation Details

Description

Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk

Part No

8B-S100-TAK

Pretreatment Note

Reconstitute with 200 µL mobile phase

Condition 1

1.0 mL Methanol

Condition 2

1.0 mL Water

Load

Load 1000.00 µL Sample

Wash 1

1.0 mL Methanol:Water

Dry

1 min @ 10 in. Hg

Elution 1

Methanol

Evaporation

Evaporate to dryness

Reconstitute

200 µL Mobile Phase