Extraction of Barbiturates from Urine on Strata™ X
LC Conditions
Column
Synergi 4 µm Max-RP 80 Å, LC Column 150 x 4.6 mm, Ea
Brand
Synergi
Part No
00F-4337-E0
Phase Name
Max-RP
Elution Type
Gradient
Mobile Phase
A: 20 mM KH2PO4, pH 7.0
B: Acetonitrile
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
80
20
2
3
80
20
3
15
50
50
4
16
80
20
Flow Rate
1.00 mL/min
Temperature
22°C
Detection
UV-Vis Abs.-Fixed Wave. (UV) @ (22°C)
Detection Info
Sample Notes
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074 - Holder Part No.: KJ0-4282
Sample Preparation Details
Description
Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk
Part No
8B-S100-TAK
Pretreatment Note
Blow down under slow stream of N2 @ 40° C and reconstitute with 300 µL water
Condition 1
1.0 mL Methanol
Condition 2
1.0 mL Water
Load
Load 1.00 mL Sample diluted in KH2PO4 (100mM) pH 6.0
Wash 1
1.0 mL Methanol / Water (5:95)
Wash 2
1.0 mL Methanol / 2% CH3COOH in Water (10:90)
Elution 1
1.0 mL Methanol / 2% Ammonium Hydroxide in Water (35:65)
Evaporation
Evaporate to dryness
Reconstitute
300 µL Water
Order Items Used in This Application
- 1. Phenobarbital
- 2. Butabarbital
- 3. Butalbital
- 4. Amobarbital
- 5. Secobarbital
-OEChem-06211003152D<br><br> 29 30 0 0 0 0 0 0 0999 V2000<br> 2.0000 0.2010 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4641 0.2010 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -2.7990 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -1.2990 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 -1.2990 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 0.2010 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.2320 1.0670 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2320 1.0670 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -0.2990 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 -0.2990 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.2320 1.0670 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 1.9330 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2320 1.0670 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -1.7990 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2320 2.7990 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.7320 1.9330 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2320 2.7990 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8147 1.2790 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.1244 1.6776 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.2320 1.6870 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.6120 1.0670 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.2320 0.4470 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3291 -1.6090 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 -1.6090 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.1120 1.9330 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.5420 0.5301 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.9220 3.3360 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3520 1.9330 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.5420 3.3360 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 9 2 0 0 0 0<br> 2 10 2 0 0 0 0<br> 3 14 2 0 0 0 0<br> 4 9 1 0 0 0 0<br> 4 14 1 0 0 0 0<br> 4 23 1 0 0 0 0<br> 5 10 1 0 0 0 0<br> 5 14 1 0 0 0 0<br> 5 24 1 0 0 0 0<br> 6 7 1 0 0 0 0<br> 6 8 1 0 0 0 0<br> 6 9 1 0 0 0 0<br> 6 10 1 0 0 0 0<br> 7 11 1 0 0 0 0<br> 7 18 1 0 0 0 0<br> 7 19 1 0 0 0 0<br> 8 12 2 0 0 0 0<br> 8 13 1 0 0 0 0<br> 11 20 1 0 0 0 0<br> 11 21 1 0 0 0 0<br> 11 22 1 0 0 0 0<br> 12 15 1 0 0 0 0<br> 12 25 1 0 0 0 0<br> 13 16 2 0 0 0 0<br> 13 26 1 0 0 0 0<br> 15 17 2 0 0 0 0<br> 15 27 1 0 0 0 0<br> 16 17 1 0 0 0 0<br> 16 28 1 0 0 0 0<br> 17 29 1 0 0 0 0<br>M END
Compound Name
Phenobarbital
CID
4763
Molecular Formula
C12H12N2O3
Molecular Weight
232
No. Hydrogen Bond Acceptors
3
No. Hydrogen Bond Donors
2
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