Anion Exchange 20mer Oligo on Luna Amino

LC Conditions

Column

Luna 5 µm NH2 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4378-E0

Phase Name

NH2

Elution Type

Gradient

Mobile Phase

A: 20mM Sodium Phosphate in 90/10 Water/Acetonitrile

B: 20mM Sodium Phosphate in 90/10 Water/Acetonitrile + 1000mM NaCl

Gradient Profile

Step No.

Time(min)

%A

%B

1

2

75

25

2

45

5

95

Flow Rate

1.00 mL/min

Temperature

40°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, NH2 4 x 3.0mm, 10/Pk Part No.: AJ0-4302 - Holder Part No.: KJ0-4282