Organic Acids on Hyperclone 5u C18

LC Conditions

Column

HyperClone 5 µm ODS (C18) 120 Å, LC Column 150 x 4.6 mm, Ea

Brand

HyperClone

Part No

00F-4361-E0

Phase Name

ODS (C18)

Elution Type

Isocratic

Mobile Phase

A: 20mM KH2PO4, pH=2.5

B: MeOH

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282