Tryptic Digest of Cytochrome c on Luna SCX

LC Conditions

Column

Luna 5 µm SCX 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4398-E0

Phase Name

SCX

Elution Type

Gradient

Mobile Phase

A: 20mM KPhos pH 2.5 + 25% Acetonitrile

B: A + 350mM KCl

Gradient Profile

Step No.

Time(min)

%A

%B

1

1

100

0

2

50

0

100

Flow Rate

1.00 mL/min

Temperature

30°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, SCX 4 x 3.0mm, 10/Pk Part No.: AJ0-4308 - Holder Part No.: KJ0-4282