Tricyclic Antidepressants at pH 11 in Gradient RP HPLC

LC Conditions

Column

Gemini 5 µm C18 110 Å, LC Column 150 x 4.6 mm, Ea

Brand

Gemini

Part No

00F-4435-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 20mM TEA, pH 11.0

B: ACN

C: MeOH

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

35

25

2

15

20

25

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 3.0mm, 10/Pk Part No.: AJ0-7597 - Holder Part No.: KJ0-4282