Steroids on Onyx C18

LC Conditions

Column

Onyx Monolithic C18, LC Column 50 x 4.6 mm, Ea

Brand

Onyx

Part No

CH0-7644

Phase Name

Monolithic C18

Elution Type

Gradient

Mobile Phase

A: Water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

3

50

50

3

4.5

50

50

Flow Rate

4.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info