Peptides (Angiotensin) on Onyx C8

LC Conditions

Column

Onyx Monolithic C8, LC Column 100 x 4.6 mm, Ea

Brand

Onyx

Part No

CH0-7647

Phase Name

Monolithic C8

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA in Water

B: 0.1% TFA in 90/10 Acetonitrile:Water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

55

45

2

1

10

90

3

3

10

90

Flow Rate

2.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info