Hydrophobic Bases at Low pH on Onyx C18

LC Conditions

Column

Onyx Monolithic C18, LC Column 100 x 4.6 mm, Ea

Brand

Onyx

Part No

CH0-7643

Phase Name

Monolithic C18

Elution Type

Isocratic

Mobile Phase

A: 20mM Phosphate buffer, pH 2.5

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

25

75

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info