Zearalone analysis using Synergi Hydro-RP

LC Conditions

Column

Synergi 4 µm Hydro-RP 80 Å, LC Column 150 x 3 mm, Ea

Brand

Synergi

Part No

00F-4375-Y0

Phase Name

Hydro-RP

Elution Type

Isocratic

Mobile Phase

A: 0.1% Formic acid in Water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

50

50

Flow Rate

0.50 mL/min

Temperature

22°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, AQ C18 4 x 2.0mm, 10/Pk Part No.: AJ0-4286 - Holder Part No.: KJ0-4282