White wine analysis using Onyx C18

LC Conditions

Column

Onyx Monolithic C18, LC Column 100 x 4.6 mm, Ea

Brand

Onyx

Part No

CH0-7643

Phase Name

Monolithic C18

Elution Type

Gradient

Mobile Phase

A: 0.1% H3PO4 in Water

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

10

97

3

2

22

82

18

4

30

50

50

5

35

50

50

Flow Rate

2.00 mL/min

Temperature

30°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info