Beta-Blockers (polar basics) at low pH by Gemini 5um C18

LC Conditions

Column

Gemini 5 µm C18 110 Å, LC Column 150 x 4.6 mm, Ea

Brand

Gemini

Part No

00F-4435-E0

Phase Name

C18

Elution Type

Isocratic

Mobile Phase

A: 20mM Phosphate buffer pH2.5

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

75

25

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

Peak 4 -- Labetolol Efficiency = 7127 Peak Symmetry = 0.68