Flavonol and Xanthone Glycosides from Mango Peels

LC Conditions

Column

Synergi 4 µm Hydro-RP 80 Å, LC Column 150 x 3 mm, Ea

Brand

Synergi

Part No

00F-4375-Y0

Phase Name

Hydro-RP

Elution Type

Gradient

Mobile Phase

A: 2% (v/v) acetic acid in water

B: 0.5% acetic acid in water and acetonitrile (50:50, v/v)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

15

75

25

3

50

70

30

4

60

20

80

5

65

0

100

6

65.5

100

0

Flow Rate

0.60 mL/min

Temperature

25°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, AQ C18 4 x 2.0mm, 10/Pk Part No.: AJ0-4286 - Holder Part No.: KJ0-4282