Anthocyanins in Red Wine

LC Conditions

Column

Synergi 4 µm Max-RP 80 Å, LC Column 250 x 4.6 mm, Ea

Brand

Synergi

Part No

00G-4337-E0

Phase Name

Max-RP

Elution Type

Gradient

Mobile Phase

A: water/acetonitril/formic acid 87:3:10

B: water/acetonitril/formic acid 40:50:10

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

94

6

2

20

80

20

3

35

60

40

4

40

40

60

5

45

10

90

6

50

10

90

Flow Rate

0.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

Courtesy of M. Schwarz, G. Hofmann, P. Winterhalter, TU Braunschweig, Institut für Lebensmittelchemie