Phospholipids in HILIC mode using Luna NH2

LC Conditions

Column

Luna 5 µm NH2 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4378-E0

Phase Name

NH2

Elution Type

Gradient

Mobile Phase

A: Water

B: Acteonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

5

95

2

15

50

50

Flow Rate

1.00 mL/min

Temperature

30°C

Detection

ELSD @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, NH2 4 x 3.0mm, 10/Pk Part No.: AJ0-4302 - Holder Part No.: KJ0-4282