Nucleosides using Synergi Fusion-RP
LC Conditions
Column
Synergi 4 µm Fusion-RP 80 Å, LC Column 150 x 4.6 mm, Ea
Brand
Synergi
Part No
00F-4424-E0
Phase Name
Fusion-RP
Elution Type
Gradient
Mobile Phase
A: 20mM Ammonium acetate pH 4.5
B: Acetonitrile
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
95
5
2
10
60
40
Flow Rate
1.00 mL/min
Temperature
22°C
Detection
UV-Vis Abs.-Variable Wave.(UV) @ (22°C)
Detection Info
Sample Notes
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Fusion-RP 4 x 3.0mm, 10/Pk Part No.: AJ0-7557 - Holder Part No.: KJ0-4282
Order Items Used in This Application
- 1. Cytidine
- 2. Uridine
- 3. Guanosine
- 4. Thymine
- 5. Adenine
-OEChem-06211003142D<br><br> 30 31 0 1 0 0 0 0 0999 V2000<br> 5.0298 -1.2857 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.4608 -0.9766 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.1330 -3.0457 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3031 -2.9412 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.4888 0.3021 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2208 0.3021 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3548 1.8021 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2208 3.3021 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4118 -1.2857 0.0000 C 0 0 3 0 0 0 0 0 0 0 0 0<br> 3.7208 -2.2367 0.0000 C 0 0 3 0 0 0 0 0 0 0 0 0<br> 4.2208 -0.6979 0.0000 C 0 0 3 0 0 0 0 0 0 0 0 0<br> 4.7208 -2.2367 0.0000 C 0 0 3 0 0 0 0 0 0 0 0 0<br> 5.3086 -3.0457 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.0868 0.8021 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3548 0.8021 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.0868 1.8021 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2208 2.3021 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8108 -1.8867 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8813 -2.1037 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4635 -0.3120 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.5604 -2.1037 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7514 -3.3175 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4795 -3.6417 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -1.3915 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3852 -3.6121 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.6238 0.4921 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.6676 -3.4428 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.6238 2.1121 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7578 3.6121 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.6839 3.6121 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 11 1 0 0 0 0<br> 1 12 1 0 0 0 0<br> 2 9 1 0 0 0 0<br> 2 24 1 0 0 0 0<br> 3 10 1 0 0 0 0<br> 3 25 1 0 0 0 0<br> 4 13 1 0 0 0 0<br> 4 27 1 0 0 0 0<br> 5 15 2 0 0 0 0<br> 6 11 1 0 0 0 0<br> 6 14 1 0 0 0 0<br> 6 15 1 0 0 0 0<br> 7 15 1 0 0 0 0<br> 7 17 2 0 0 0 0<br> 8 17 1 0 0 0 0<br> 8 29 1 0 0 0 0<br> 8 30 1 0 0 0 0<br> 9 10 1 0 0 0 0<br> 9 11 1 0 0 0 0<br> 9 18 1 0 0 0 0<br> 10 12 1 0 0 0 0<br> 10 19 1 0 0 0 0<br> 11 20 1 0 0 0 0<br> 12 13 1 0 0 0 0<br> 12 21 1 0 0 0 0<br> 13 22 1 0 0 0 0<br> 13 23 1 0 0 0 0<br> 14 16 2 0 0 0 0<br> 14 26 1 0 0 0 0<br> 16 17 1 0 0 0 0<br> 16 28 1 0 0 0 0<br>M END
Compound Name
Cytidine
CID
596
Molecular Formula
C9H13N3O5
Molecular Weight
243
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
4
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