Aromatic Acids (non-polar acids) at low pH by Luna 5um C18(2)

LC Conditions

Column

Luna 5 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4252-E0

Phase Name

C18(2)

Elution Type

Isocratic

Mobile Phase

A: 20mM Phosphate buffer pH2.5

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

75

25

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

Peak 3 (salicylic acid) N = 11442, Symm = 0.68, Peak area = 4033