20nt DNA Oligo-RP Purification - 20 Minute Dry Out

LC Conditions

Column

Clarity 3 µm Oligo-RP, LC Column 50 x 4.6 mm, Ea

Brand

Clarity LC

Part No

00B-4441-E0

Phase Name

Oligo-RP

Elution Type

Gradient

Mobile Phase

A: 50mM TEAA/ 5%ACN

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

20

40

60

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

Post conditioning 20 minute vacuum drying (compare with control sample AppID 15804)

Sample Preparation Details

Condition 1

1.5 mL Methanol/Water (1:1) at 1 mL/min

Condition 2

6 mL Triophilic Buffer at 1 mL/min

Load

Load 1.50 mL Sample diluted in Triophilic Buffer

Wash 1

8 mL Triophilic Buffer at 1 mL/min

Wash 2

3 mL 2% TFA/50mM NaCl at 1 mL/min

Wash 3

4 mL Water at 1 mL/min

Elution 1

1 mL 40%Methanol/40%Water/20%Acetonitrile at 1 mL/min

Evaporation

Evaporate to dryness