Saccharin analysis using Synergi Polar-RP

LC Conditions

Column

Synergi 4 µm Polar-RP 80 Å, LC Column 150 x 4.6 mm, Ea

Brand

Synergi

Part No

00F-4336-E0

Phase Name

Polar-RP

Elution Type

Gradient

Mobile Phase

A: Water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

15

50

50

Flow Rate

1.00 mL/min

Temperature

30°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (100°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, POLAR-RP 4 x 3.0mm, 10/Pk Part No.: AJ0-6076 - Holder Part No.: KJ0-4282