Basic Compounds at High pH: Gemini 10u C18 Axia Packed 50x21.2mm vs. Competitive 5u Column 50x19mm
LC Conditions
Column
Gemini 10 µm C18 110 Å, LC Column 50 x 21.2 mm, AXIA Packed, Ea
Brand
Gemini
Part No
00B-4436-P0-AX
Phase Name
C18
Elution Type
Gradient
Mobile Phase
A: 95:5 50mM NH4HCO3 (pH 10.0) / Acetonitrile
B: 5:95 50mM NH4HCO3 (pH 10.0) / Acetonitrile
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
100
0
2
7
0
100
Flow Rate
30.00 mL/min
Temperature
22°C
Detection
UV-Vis Abs.-Variable Wave.(UV) @ (22°C)
Detection Info
Sample Notes
Comparison of Gemini 10µm C18 Axia Packed column vs. leading 5µm competitive column. Flow rates adjusted to yield the same linear velocity. Contact your Technical Representative for more details.
Order Items Used in This Application
- 1. Methacycline
- 2. Triprolidine
- 3. Amitriptyline
-OEChem-05081000432D<br><br> 54 57 0 1 0 0 0 0 0999 V2000<br> 6.0678 1.5754 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9338 -1.9246 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9338 2.0754 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.6839 1.9818 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4037 -0.9488 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6822 2.1100 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4075 2.0995 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.5194 2.1530 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1854 -1.9592 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5357 0.6029 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0678 -0.4246 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 6.0678 0.5754 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 6.9338 -0.9246 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 7.7998 -0.4246 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 5.1738 -0.9593 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 7.7998 0.5754 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9338 1.0754 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1738 1.1100 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2678 -0.4455 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6938 -0.9593 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2678 0.5962 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6938 1.1100 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.5998 -0.4455 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.5998 0.5962 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3252 -2.4692 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0572 -2.4492 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6822 -1.9592 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4037 1.0995 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.5310 -1.0024 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.5310 1.1531 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.4748 -0.4671 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.4748 0.6179 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0743 -1.2746 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3969 -1.2346 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.7933 -1.2746 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.7149 -1.2620 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.7578 2.1123 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.4707 -2.2346 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.0090 -1.9359 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7919 -2.7854 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.6414 -3.0025 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.7534 -2.9897 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.5976 -2.7530 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3609 -1.9087 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.1417 -2.2630 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.2155 -2.2754 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.5238 -1.6223 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.2155 2.4261 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.0105 -0.7792 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.0105 0.9299 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 0.9149 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5334 -0.0171 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8718 2.4116 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.0528 2.4692 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12 1 1 6 0 0 0<br> 1 37 1 0 0 0 0<br> 13 2 1 6 0 0 0<br> 2 38 1 0 0 0 0<br> 3 17 2 0 0 0 0<br> 4 18 2 0 0 0 0<br> 5 19 2 0 0 0 0<br> 6 22 1 0 0 0 0<br> 6 48 1 0 0 0 0<br> 7 28 1 0 0 0 0<br> 7 53 1 0 0 0 0<br> 8 30 1 0 0 0 0<br> 8 54 1 0 0 0 0<br> 15 9 1 6 0 0 0<br> 9 25 1 0 0 0 0<br> 9 26 1 0 0 0 0<br> 10 28 1 0 0 0 0<br> 10 51 1 0 0 0 0<br> 10 52 1 0 0 0 0<br> 11 12 1 0 0 0 0<br> 11 13 1 0 0 0 0<br> 11 15 1 0 0 0 0<br> 11 33 1 6 0 0 0<br> 12 17 1 0 0 0 0<br> 12 18 1 0 0 0 0<br> 13 14 1 0 0 0 0<br> 13 34 1 0 0 0 0<br> 14 16 1 0 0 0 0<br> 14 20 1 0 0 0 0<br> 14 35 1 6 0 0 0<br> 15 19 1 0 0 0 0<br> 15 36 1 0 0 0 0<br> 16 17 1 0 0 0 0<br> 16 22 2 0 0 0 0<br> 18 21 1 0 0 0 0<br> 19 21 1 0 0 0 0<br> 20 23 1 0 0 0 0<br> 20 27 2 0 0 0 0<br> 21 28 2 0 0 0 0<br> 22 24 1 0 0 0 0<br> 23 24 2 0 0 0 0<br> 23 29 1 0 0 0 0<br> 24 30 1 0 0 0 0<br> 25 39 1 0 0 0 0<br> 25 40 1 0 0 0 0<br> 25 41 1 0 0 0 0<br> 26 42 1 0 0 0 0<br> 26 43 1 0 0 0 0<br> 26 44 1 0 0 0 0<br> 27 45 1 0 0 0 0<br> 27 46 1 0 0 0 0<br> 29 31 2 0 0 0 0<br> 29 47 1 0 0 0 0<br> 30 32 2 0 0 0 0<br> 31 32 1 0 0 0 0<br> 31 49 1 0 0 0 0<br> 32 50 1 0 0 0 0<br>M END
Compound Name
Methacycline
CID
5281054
Molecular Formula
C22H22N2O8
Molecular Weight
442
No. Hydrogen Bond Acceptors
10
No. Hydrogen Bond Donors
6
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