OPA Amino Acids on Gemini C18
LC Conditions
Column
Gemini 5 µm C18 110 Å, LC Column 150 x 4.6 mm, Ea
Brand
Gemini
Part No
00F-4435-E0
Phase Name
C18
Elution Type
Gradient
Mobile Phase
A: 40mM NaH2PO4, pH 7.8
B: Acetonitrile/Methanol/Water (45:45:10)
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
100
0
2
40.5
59.5
40.5
3
41
39
61
4
43
39
61
5
44
18
82
6
44.5
0
100
7
46.5
0
100
8
47
100
0
9
49
100
0
Flow Rate
1.00 mL/min
Temperature
40°C
Detection
Fluorimetric @ (22°C)
Detection Info
Sample Notes
Courtesy of Jens Olaf Kroemer, Technische Biochemie, Universität des Saarlandes, Germany
Order Items Used in This Application
- 1. Aspartic acid
- 2. Glutamic acid
- 3. Cysteine
- 4. Asparagine
- 5. Serine
- 6. Homocysteine
- 7. Glutamine
- 8. Histidine
- 9. Homoserin
- 10. Glycine
- 11. Threonine
- 12. Citrulline
- 13. Arginine
- 14. beta-Alanine
- 15. Alanine
- 16. gamma-Aminobutyric acid
- 17. o-Acetyl-serine
- 18. Tyrosine
- 19. o-Acetyl-homoserine
- 20. alpha-Aminobutyric acid
- 21. L-Cystathionine
- 22. Histidine 2
- 23. Valine
- 24. Methionine
- 25. Norvaline
- 26. Tryptophan
- 27. Phenylalanine
- 28. Isoleucine
- 29. Ornithine
- 30. Leucine
- 31. Lysine
- 32. Proline
Similar Applications
No Similar applications found.