Non-Polar Basic Drugs by Gemini 5u C6-Phenyl

LC Conditions

Column

Gemini 5 µm C6-Phenyl 110 Å, LC Column 150 x 4.6 mm, Ea

Brand

Gemini

Part No

00F-4444-E0

Phase Name

C6-Phenyl

Elution Type

Isocratic

Mobile Phase

A: 20mM Potassium phosphate buffer, pH 2.5

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

55

45

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

N (diphenyhydramine) = 5533 SYMM (diphenhydramine) = 0.65 Backpressure = 217 bar