MercuryMS - Luna 3µm C18(2) 20 x 2.0mm for Benzodiazepines in Plasma by LC/MS/MS

LC Conditions

Column

Luna 3 µm C18(2) 100 Å, LC Column 20 x 2 mm, MercuryMS, Ea

Brand

Luna

Part No

00M-4251-B0-CE

Phase Name

C18(2)

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: 0.1% Formic Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

1

5

95

3

1.01

85

15

4

2

85

15

Flow Rate

0.60 mL/min

Temperature

22°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C8 4 x 2.0mm, 10/Pk Part No.: AJ0-4289 - Holder Part No.: KJ0-4282

Sample Preparation Details

Description

Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk

Part No

8B-S100-TAK

Condition 1

1 mL Methanol at 1 mL/min

Condition 2

1 mL Water at 1 mL/min

Load

Load 1.00 mL Sample

Wash 1

1 mL Water at 1.5 mL/min

Wash 2

1 mL 20% MeOH in water at 1.5 mL/min

Elution 1

1 mL Methanol at 0.5 mL/min

Evaporation

Evaporate to dryness

Reconstitute

200 µL 15% CH3CN in water