MercuryMS - Luna 3µm C18(2) 20 x 2.0mm (mini column) for Tricyclics in Plasma by LC/MS/MS

LC Conditions

Column

Luna 3 µm C18(2) 100 Å, LC Column 20 x 2 mm, Ea

Brand

Luna

Part No

00M-4251-B0

Phase Name

C18(2)

Elution Type

Isocratic

Mobile Phase

A: 0.1% Formic Acid in Water

B: 0.1% Formic Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

28

72

Flow Rate

0.60 mL/min

Temperature

22°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Notes

Total Ion Chromatogram is shown below. Single Ion Chromatograms are available. Please ask your Phenomenex Technical Representative.

Sample Preparation Details

Description

Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 1 mL, Tubes, 100/Pk

Part No

8B-S029-TAK

Condition 1

1 mL Methanol at 1 mL/min

Condition 2

1 mL Water at 1 mL/min

Load

Load 1.00 mL Sample

Wash 1

1 mL 0.1M acetic acid at 1.5 mL/min

Wash 2

2 mL MeOH at 1.5 mL/min

Elution 1

1 mL 5% ammonium hydroxide(28-30%) in MeOH at 0.5 mL/min

Evaporation

Evaporate to dryness

Reconstitute

200 µL Mobile Phase