Separation of 39 and 40mer DNA Mix

LC Conditions

Column

Clarity 3 µm Oligo-RP, LC Column 50 x 4.6 mm, Ea

Brand

Clarity LC

Part No

00B-4441-E0

Phase Name

Oligo-RP

Elution Type

Gradient

Mobile Phase

A: 50 mm TEAA, pH 7.5

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

75

25

2

30

65

35

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Clarity Oligo-RP 4 x 3.0mm, 10/Pk Part No.: AJ0-8135 - Holder Part No.: KJ0-4282