Steroids on Gemini 5µm C18 250 x 3mm ID

LC Conditions

Column

Gemini 5 µm C18 110 Å, LC Column 250 x 3 mm, Ea

Brand

Gemini

Part No

00G-4435-Y0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: 0.1% Formic Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

60

40

2

4

60

40

3

15

25

75

4

15.1

60

40

5

20

60

40

Flow Rate

0.60 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 2.0mm, 10/Pk Part No.: AJ0-7596 - Holder Part No.: KJ0-4282