Prep Scale Cartridge Purification of Phosphorothioate DNA

LC Conditions

Column

Clarity 3 µm Oligo-RP, LC Column 50 x 4.6 mm, Ea

Brand

Clarity LC

Part No

00B-4441-E0

Phase Name

Oligo-RP

Elution Type

Gradient

Mobile Phase

A: 50M TEAA/5% Acetonitrile

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

20

25

75

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Clarity Oligo-RP 4 x 3.0mm, 10/Pk Part No.: AJ0-8135 - Holder Part No.: KJ0-4282

Sample Preparation Details

Condition 1

10 mL Methanol/Water at 1 mL/min

Condition 2

20 mL Triophilic Buffer at 1 mL/min

Load

Load 50.00 mL Sample diluted in 100mM TEAA

Wash 1

40 mL 100mM TEAA/10% Acetonitrile/20% Methanol at 1 mL/min

Elution 1

Methanol/Acetonitrile/Water

Evaporation

Evaporate to dryness