Simultaneous Desalting and Selective Elution of Basic, Neutral and Acidic Peptides

LC Conditions

Column

Synergi 4 µm Max-RP 80 Å, LC Column 150 x 4.6 mm, Ea

Brand

Synergi

Part No

00F-4337-E0

Phase Name

Max-RP

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA in Water

B: 0.1% TFA in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

12

35

65

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074 - Holder Part No.: KJ0-4282

Sample Preparation Details

Description

Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk

Part No

8B-S100-TAK

Condition 1

1 mL Acetonitrile

Condition 2

Water

Load

Load 840.00 µL Sample diluted in water with salt

Wash 1

2 mL Water

Elution 1

0.25 mL 40:60 Acetonitrile/Water. for acidic peptides

Elution 2

0.25 mL 70:30 Acetonitrile/Water. for neutral peptides

Elution 3

0.25 mL 70:30 Acetonitrile/1.0% Formic Acid in Water. for basic peptides

Evaporation

Evaporate to dryness

Reconstitute

20 µL initial mobile phase