Antibiotics

LC Conditions

Column

Luna 5 µm C18(2) 100 Å, LC Column 250 x 4.6 mm, Ea

Brand

Luna

Part No

00G-4252-E0

Phase Name

C18(2)

Elution Type

Isocratic

Mobile Phase

A: Acetonitrile

B: Buffer (13.1g K2HPO4x3H2O + 0.3g KH2PO4 + 1.5g sodium heptanesulfonate + 500mL H2O)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

60

40

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282