PEGylated Insulin on Jupiter® 300 C4, C18 and Two Experimental Columns

LC Conditions

Column

Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea

Brand

Jupiter

Part No

00F-4167-E0

Phase Name

C4

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA and 2% ACN in Water

B: 70/20% ACN/IPA, 0.08% TFA in Water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

25

30

70

Flow Rate

1.00 mL/min

Temperature

45°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

Reversed phase of PEGylated Insulin run on listed columns. Note the reduced resolution for the Jupiter C18 and EXP 2 columns when compared to the Jupiter C4 column. Although it is difficult to visualize, the Jupiter actully has better resolution of PEGylated forms than the EXP1 column.