Jupiter® 300 C4 - Effect of Temperature on PEGylated Protein Separations

LC Conditions

Column

Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea

Brand

Jupiter

Part No

00F-4167-E0

Phase Name

C4

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA in Water

B: 90% ACN 0.09% TFA in Water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

25

45

55

Flow Rate

1.00 mL/min

Temperature

45°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

Reversed phase runs of PEGylated carbonic anhydrase at different temperatures. Increasing the temperature to 45°C slightly improves resolution of different PEG forms.