Acid, Base, Neutral Test Mix on Synergi 2.5 um Polar-RP-HST

LC Conditions

Column

Synergi 2.5 µm Polar-RP 100 Å, LC Column 50 x 2 mm, Ea

Brand

Synergi

Part No

00B-4371-B0

Phase Name

Polar-RP

Elution Type

Gradient

Mobile Phase

A: 0.1 % Formic acid in Water

B: 0.1 % Formic Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

2.9

5

95

Flow Rate

1.10 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info