Carboxypeptitase B treated murine myeloma IgG1-k

LC Conditions

Column

Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea

Brand

Jupiter

Part No

00F-4167-E0

Phase Name

C4

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA and 2% Acetonitrile in Water

B: 70% Acetonitrile, 20% Isopropanol, and 0.08% TFA in Water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

20

10

90

3

21

80

20

4

25

80

20

Flow Rate

1.00 mL/min

Temperature

55°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (55°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Widepore C4 4 x 3.0mm, 10/Pk Part No.: AJ0-4330 - Holder Part No.: KJ0-4282