Nicotine and Metabolites on Luna 5um HILIC
LC Conditions
Column
Luna 5 µm HILIC 200 Å, LC Column 100 x 2 mm, Ea
Brand
Luna
Part No
00D-4450-B0
Phase Name
HILIC
Elution Type
Isocratic
Mobile Phase
A: 90/10 Acetonitrile/100mM Ammonium Formate pH 3.2
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
100
0
Flow Rate
0.40 mL/min
Temperature
22°C
Detection
Mass Spectrometer (MS) @ (22°C)
Detection Info
Sample Notes
The pH of the aqueous buffer is specified. The pH in mixed organic was not measured.
Order Items Used in This Application
- 1. Cotinine
- 2. Nicotine
- 3. Nornicotine
-OEChem-04281015372D<br><br> 25 26 0 1 0 0 0 0 0999 V2000<br> 2.6723 2.6739 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.9511 0.9139 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.6261 -2.1739 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7601 0.3261 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 4.5691 0.9139 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2601 1.8649 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.2601 1.8649 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7601 -0.6739 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 0.6048 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8940 -1.1739 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.6261 -1.1739 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8940 -2.1739 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7601 -2.6739 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3125 0.0446 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8791 0.3769 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1355 1.1660 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8665 1.9938 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.1953 2.4815 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.8084 1.1945 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4103 0.4132 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.1916 0.0152 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3571 -0.8639 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1630 -0.8639 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3571 -2.4839 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7601 -3.2939 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 7 2 0 0 0 0<br> 2 4 1 0 0 0 0<br> 2 7 1 0 0 0 0<br> 2 9 1 0 0 0 0<br> 3 11 1 0 0 0 0<br> 3 13 2 0 0 0 0<br> 4 5 1 0 0 0 0<br> 4 8 1 1 0 0 0<br> 4 14 1 0 0 0 0<br> 5 6 1 0 0 0 0<br> 5 15 1 0 0 0 0<br> 5 16 1 0 0 0 0<br> 6 7 1 0 0 0 0<br> 6 17 1 0 0 0 0<br> 6 18 1 0 0 0 0<br> 8 10 1 0 0 0 0<br> 8 11 2 0 0 0 0<br> 9 19 1 0 0 0 0<br> 9 20 1 0 0 0 0<br> 9 21 1 0 0 0 0<br> 10 12 2 0 0 0 0<br> 10 22 1 0 0 0 0<br> 11 23 1 0 0 0 0<br> 12 13 1 0 0 0 0<br> 12 24 1 0 0 0 0<br> 13 25 1 0 0 0 0<br>M END
Compound Name
Cotinine
CID
854019
Molecular Formula
C10H12N2O
Molecular Weight
176
No. Hydrogen Bond Acceptors
2
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