Ribavirin on Luna 5µm HILIC
LC Conditions
Column
Luna 5 µm HILIC 200 Å, LC Column 100 x 2 mm, Ea
Brand
Luna
Part No
00D-4450-B0
Phase Name
HILIC
Elution Type
Gradient
Mobile Phase
A: 90/10 Acetonitrile/100mM Ammonium Formate pH 3.2
B: 50/50 Acetonitrile/20mM Ammonium Formate pH 3.2
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
100
0
2
1
100
0
3
3
0
100
4
4.5
0
100
5
4.51
100
0
6
9.51
100
0
Flow Rate
0.40 mL/min
Temperature
22°C
Detection
Mass Spectrometer (MS) @ (22°C)
Detection Info
Sample Notes
The pH of the aqueous buffer is specified. The pH in mixed organic was not measured.
Order Items Used in This Application
- 1. Ribavirin
-OEChem-05081000432D<br><br> 29 30 0 1 0 0 0 0 0999 V2000<br> 5.0298 -1.5122 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.4608 -1.2031 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.1330 -3.2722 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3031 -3.1677 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3031 2.3190 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2208 0.0756 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.0298 0.6634 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7208 1.6145 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.9019 3.3370 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7208 -2.4632 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 3.4118 -1.5122 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 4.7208 -2.4632 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 4.2208 -0.9244 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 5.3086 -3.2722 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4118 0.6634 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7208 1.6145 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.3086 2.4235 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3148 -0.8998 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.1085 -2.3662 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.3332 -2.3662 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.6684 -0.6429 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7514 -3.5440 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4795 -3.8682 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -1.6180 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3852 -3.8386 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8222 0.4718 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.6676 -3.6693 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2663 3.8386 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2853 3.4018 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 12 1 0 0 0 0<br> 1 13 1 0 0 0 0<br> 11 2 1 1 0 0 0<br> 2 24 1 0 0 0 0<br> 10 3 1 1 0 0 0<br> 3 25 1 0 0 0 0<br> 4 14 1 0 0 0 0<br> 4 27 1 0 0 0 0<br> 5 17 2 0 0 0 0<br> 6 7 1 0 0 0 0<br> 13 6 1 6 0 0 0<br> 6 15 1 0 0 0 0<br> 7 16 2 0 0 0 0<br> 8 15 2 0 0 0 0<br> 8 16 1 0 0 0 0<br> 9 17 1 0 0 0 0<br> 9 28 1 0 0 0 0<br> 9 29 1 0 0 0 0<br> 10 11 1 0 0 0 0<br> 10 12 1 0 0 0 0<br> 10 19 1 0 0 0 0<br> 11 13 1 0 0 0 0<br> 11 18 1 0 0 0 0<br> 12 14 1 6 0 0 0<br> 12 20 1 0 0 0 0<br> 13 21 1 0 0 0 0<br> 14 22 1 0 0 0 0<br> 14 23 1 0 0 0 0<br> 15 26 1 0 0 0 0<br> 16 17 1 0 0 0 0<br>M END
Compound Name
Ribavirin
CID
37542
Molecular Formula
C8H12N4O5
Molecular Weight
244
No. Hydrogen Bond Acceptors
7
No. Hydrogen Bond Donors
4
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