Morphine and Metabolites on Gemini 5µm C18

LC Conditions

Column

Gemini 5 µm C18 110 Å, LC Column 100 x 2 mm, Ea

Brand

Gemini

Part No

00D-4435-B0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% v/v Formic Acid

B: Acetonitrile with 0.1% v/v Formic Acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

1

97

3

3

3

70

30

4

3.01

97

3

5

5.5

97

3

Flow Rate

0.40 mL/min

Temperature

22°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Notes

The pH of the aqueous acid is specified.