Nicotine and Metabolites on Gemini 5µm C18
LC Conditions
Column
Gemini 5 µm C18 110 Å, LC Column 100 x 2 mm, Ea
Brand
Gemini
Part No
00D-4435-B0
Phase Name
C18
Elution Type
Gradient
Mobile Phase
A: Water with 0.1% v/v Formic Acid
B: Acetonitrile with 0.1% v/v Formic Acid
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
97
3
2
1
97
3
3
3
70
30
4
3.01
97
3
5
5.5
97
3
Flow Rate
0.40 mL/min
Temperature
22°C
Detection
Mass Spectrometer (MS) @ (22°C)
Detection Info
Sample Notes
The pH of the aqueous acid is 2.7. 0.5ng on-column injection of each analyte.
Order Items Used in This Application
- 1. Nornicotine
- 2. Nicotine
- 3. Cotinine
-OEChem-06211003142D<br><br> 23 24 0 1 0 0 0 0 0999 V2000<br> 2.5896 1.3184 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2647 -1.7694 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3987 0.7306 0.0000 C 0 0 3 0 0 0 0 0 0 0 0 0<br> 4.2077 1.3184 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8987 2.2694 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8987 2.2694 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3987 -0.2694 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5326 -0.7694 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2647 -0.7694 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5326 -1.7694 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3987 -2.2694 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.9511 0.4491 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5177 0.7814 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7741 1.5705 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5051 2.3983 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8339 2.8860 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.9635 2.8860 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.2922 2.3983 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 1.1268 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.9957 -0.4594 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8016 -0.4594 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.9957 -2.0794 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3987 -2.8894 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 3 1 0 0 0 0<br> 1 6 1 0 0 0 0<br> 1 19 1 0 0 0 0<br> 2 9 1 0 0 0 0<br> 2 11 2 0 0 0 0<br> 3 4 1 0 0 0 0<br> 3 7 1 0 0 0 0<br> 3 12 1 0 0 0 0<br> 4 5 1 0 0 0 0<br> 4 13 1 0 0 0 0<br> 4 14 1 0 0 0 0<br> 5 6 1 0 0 0 0<br> 5 15 1 0 0 0 0<br> 5 16 1 0 0 0 0<br> 6 17 1 0 0 0 0<br> 6 18 1 0 0 0 0<br> 7 8 1 0 0 0 0<br> 7 9 2 0 0 0 0<br> 8 10 2 0 0 0 0<br> 8 20 1 0 0 0 0<br> 9 21 1 0 0 0 0<br> 10 11 1 0 0 0 0<br> 10 22 1 0 0 0 0<br> 11 23 1 0 0 0 0<br>M END
Compound Name
Nornicotine
CID
412
Molecular Formula
C9H12N2
Molecular Weight
148
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
1
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