Levofloxacin and Ciprofloxacin on Luna 3µm HILIC

LC Conditions

Column

Luna 3 µm HILIC 200 Å, LC Column 100 x 2 mm, Ea

Brand

Luna

Part No

00D-4449-B0

Phase Name

HILIC

Elution Type

Isocratic

Mobile Phase

A: Acetonitrile

B: 100mM Ammonium Acetate, pH 5.8

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

Flow Rate

0.40 mL/min

Temperature

22°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Notes

The pH of the aqueous buffer is specified. The pH in mixed organic was not measured.