Water-Soluble Vitamin Mixture on Luna 5µm HILIC, pH 3.2

LC Conditions

Column

Luna 5 µm HILIC 200 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4450-E0

Phase Name

HILIC

Elution Type

Gradient

Mobile Phase

A: Acetonitrile

B: Water

C: 100mM Ammonium Formate pH 3.2

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

5

2

2.5

90

5

3

10

50

45

4

12.5

50

45

Flow Rate

2.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, HILIC 4 x 3.0 mm, 10/Pk Part No.: AJ0-8329 - Holder Part No.: KJ0-4282