Insulin Mixture on Jupiter 5u C18

LC Conditions

Column

Jupiter 5 µm C18 300 Å, LC Column 150 x 4.6 mm, Ea

Brand

Jupiter

Part No

00F-4053-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA and 5% Acetonitrile in Water

B: 0.08% TFA , 90% Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

70

30

2

15

68

32

Flow Rate

1.00 mL/min

Temperature

25°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (25°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Widepore C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4321 - Holder Part No.: KJ0-4282