Glycols in Toothpaste Sample on ZB-WAXplus

GC Conditions

Column

Zebron ZB-WAXplus, GC Cap. Column 30 m x 0.25 mm x 0.25 µm, Ea

Part No

7HG-G013-11

Phase Name

ZB-WAXplus

Injection

Split 20:1 @ 225°C

Carrier Gas

Constant Flow Helium, 1.5 mL/min

Detection

Mass Spectrometer (MS) @ (250°C)

Detection Info

Sample Notes

0.10 mg toothpaste in 1.0 mL of 50-50 ACN/Water. Mixed then centrifuged. 100 µl supernatant diluted to 400 µl 50-50 ACN/Water. To inject, transfer 250 µl to an insert in sample vial with 25 µl 5mg/ml internal standard. Used a 5 m retention gap pre-column. Glycol standards are in application 16507. Note - no glycols were found in this toothpaste sample.