Sterols from Peanut Butter on ZB-5MS

GC Conditions

Column

Zebron ZB-5MS, GC Cap. Column 30 m x 0.25 mm x 0.25 µm, Ea

Part No

7HG-G010-11

Phase Name

ZB-5MS

Injection

Splitless @ 320°C

Carrier Gas

Constant Flow Helium, 2.5 mL/min

Detection

Flame Ionization (FID) @ (320°C)

Detection Info

Sample Notes

Sample prep- Saponification, Solid Phase Extraction, and Derivatization.

Sample Preparation Details

Pretreatment Note

Initial Samole Unit: gram

Condition 1

5.0 mL Methanol

Condition 2

5.0 mL Chloroform

Load

Load 0.73 mL Sample diluted in 20 ml 0.5 M KOH in Ethanol

Elution 1

20 ml 0.5 M KOH in Ethanol

Evaporation

Evaporate to dryness

Reconstitute

200 µL Pyridine