Sterols pH from Olive Oil on ZB-5MS

GC Conditions

Column

Zebron ZB-5MS, GC Cap. Column 30 m x 0.25 mm x 0.25 µm, Ea

Part No

7HG-G010-11

Phase Name

ZB-5MS

Injection

Splitless @ 275°C

Carrier Gas

Constant Flow Helium, 1.5 mL/min

Detection

Mass Spectrometer (MS) @ (275°C)

Detection Info

Sample Preparation Details

Pretreatment Note

Initial Sample Weight / Unit: 0.70 grams

Condition 1

5.0 mL Methanol

Condition 2

5.0 mL Chloroform

Load

Load Sample

Elution 1

15 ml 5 % Methanol in Chloroform

Evaporation

Evaporate to dryness