Mixtures of Acids, Neutrals and Bases in Potassium Phosphate pH 2.5 on Gemini-NX 5um C18

LC Conditions

Column

Gemini 5 µm NX-C18 110 Å, LC Column 150 x 4.6 mm, Ea

Brand

Gemini

Part No

00F-4454-E0

Phase Name

NX-C18

Elution Type

Gradient

Mobile Phase

A: 20mM Potassium Phosphate pH2.5

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

8

20

80

3

10

20

80

4

10.1

95

5

5

13

95

5

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282