Nicotine in Ammonium Bicarbonate pH 10.5 on Gemini-NX 5µ
LC Conditions
Column
Gemini 5 µm NX-C18 110 Å, LC Column 150 x 4.6 mm, Ea
Brand
Gemini
Part No
00F-4454-E0
Phase Name
NX-C18
Elution Type
Gradient
Mobile Phase
A: 10 mM Ammonium Bicarbonate pH 10.5
B: Acetonitrile
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
95
5
2
10
5
95
3
12
5
95
Flow Rate
1.50 mL/min
Temperature
22°C
Detection
UV-Vis Abs.-Variable Wave.(UV) @ (22°C)
Detection Info
Sample Notes
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282
Order Items Used in This Application
- 1. Nicotine
-OEChem-04281015272D<br><br> 26 27 0 1 0 0 0 0 0999 V2000<br> 2.9511 1.3184 0.0000 N 0 0 3 0 0 0 0 0 0 0 0 0<br> 4.6261 -1.7694 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7601 0.7306 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 4.5691 1.3184 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2601 2.2694 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.2601 2.2694 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7601 -0.2694 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 1.0094 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8940 -0.7694 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.6261 -0.7694 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8940 -1.7694 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7601 -2.2694 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3125 0.4491 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8791 0.7814 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1355 1.5705 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8665 2.3983 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.1953 2.8860 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3249 2.8860 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.6536 2.3983 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.8084 1.5990 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4103 0.8178 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.1916 0.4197 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3571 -0.4594 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1630 -0.4594 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3571 -2.0794 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7601 -2.8894 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 3 1 0 0 0 0<br> 1 6 1 0 0 0 0<br> 1 8 1 0 0 0 0<br> 2 10 1 0 0 0 0<br> 2 12 2 0 0 0 0<br> 3 4 1 0 0 0 0<br> 3 7 1 1 0 0 0<br> 3 13 1 0 0 0 0<br> 4 5 1 0 0 0 0<br> 4 14 1 0 0 0 0<br> 4 15 1 0 0 0 0<br> 5 6 1 0 0 0 0<br> 5 16 1 0 0 0 0<br> 5 17 1 0 0 0 0<br> 6 18 1 0 0 0 0<br> 6 19 1 0 0 0 0<br> 7 9 1 0 0 0 0<br> 7 10 2 0 0 0 0<br> 8 20 1 0 0 0 0<br> 8 21 1 0 0 0 0<br> 8 22 1 0 0 0 0<br> 9 11 2 0 0 0 0<br> 9 23 1 0 0 0 0<br> 10 24 1 0 0 0 0<br> 11 12 1 0 0 0 0<br> 11 25 1 0 0 0 0<br> 12 26 1 0 0 0 0<br>M END
Compound Name
Nicotine
CID
89594
Molecular Formula
C10H14N2
Molecular Weight
162
No. Hydrogen Bond Acceptors
2
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