Polar bases (beta blockers) at high pH (ammonium bicarbonate pH 10.5) on Gemini-NX 5µ

LC Conditions

Column

Gemini 5 µm NX-C18 110 Å, LC Column 150 x 4.6 mm, Ea

Brand

Gemini

Part No

00F-4454-E0

Phase Name

NX-C18

Elution Type

Gradient

Mobile Phase

A: 10mM Ammonium Bicarbonate pH 10.5

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

15

70

30

3

20

50

50

4

25

50

50

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282