Insulin on Gemini-NX 5u C18

LC Conditions

Column

Gemini 5 µm NX-C18 110 Å, LC Column 250 x 4.6 mm, Ea

Brand

Gemini

Part No

00G-4454-E0

Phase Name

NX-C18

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA in Water

B: 0.1% TFA in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

3

90

10

3

10

40

60

4

15

40

60

Flow Rate

1.00 mL/min

Temperature

28°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini-NX C18 4 x 3.0mm, 10/Pk Part No.: AJ0-8368 - Holder Part No.: KJ0-4282